Anatomical, imaging and histological observations of the circumferential full-length of superficial fascia structure of the upper limb / 中华整形外科杂志

Objective@#To describe the general and histological features of the full-length superficial fascia of the circumferential upper limb.@*Methods@#Fresh frozen arm specimens were dissected, and then MRI imaging in vivo, enhanced CT angiography and HE histological staining were used to describe the characteristics of the full-length superficial fascia of the circumferential arm and its relationship with important blood vessels.@*Results@#The four typical structures of the superficial fascia of the arm were divided into subcutaneous superficial fat, membrane-like substance, deep fat and deep fascia from superficial to deep. The thickness and stratification, fusion degree and histological characteristics of the superficial fascia of these four layers were obviously different in different levels and regions of the arm. MRI confirmed that the total thickness of superficial fascia gradually decreased from shoulder to wrist. Venography showed that the cephalic vein ran below the second layer of superficial fascia and above the deep fascia. The basilic vein originated from the dorsal vein network of the hand and always lied below the second layer of membranous material until the basilic vein penetrates below the deep fascia of the upper arm.@*Conclusions@#The deep understanding of the circumferential full-length of superficial fascia structure of the upper limb provides an important theoretical basis for improving the surgical safety and fine operation for the Dynamic Arm Circumferential Liposuction.

Comparative study on the intraocular pressure measurement with three tonometer / 中国实用护理杂志

Objective To investigate the clinical efficacy of ICARE rebounder tonometer (RBT) by comparing the measurement results of ICARE RBT,applanation tonometer (AT) and non-contact tonometer (NCT).Methods This study included 100 glaucoma suspect cases (100 eyes) with their day and night intraocular pressure (IOP) measurement in Beijing Tongren Hospital from May to November,2014.IOP of each eye was measured by two experienced examiners using RBT,AT and NCT for three times respectively and the mean values were recorded as the final result.Results IOP was measured by RBT,AT,NCT,the values were (19.1s5.7),(18.9±5.0),(15.2±5.0) mmHg (1 mmHg=0.133 kPa),respectively.The difference between RBT and AT was (0.2±1.8) mmHg,and the difference between NCT and AT was-(3.8±2.6) mmHg.There was no significant difference in the values between RBT and AT,t=-1.136,P＞0.05.There was significant difference in the values between NCT and AT,t=14.714,P＜0.01.The results measured by RBT,NCT and AT had correlation,r=0.948,0.822;P＜0.01.The relevant value was 0.973 in two examiners with RBT,which consisted with the result with AT.Conclusion RBT meets the clinical needs and can be widely used in clinical work.

Inhibition of retinoblastoma binding protein 2 promotes osteogenic differentiation of human adipose-derived stromal cells / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To explore the effect of retinoblastoma binding protein 2 (RBP-2), a histone H3K4 demethylase, on osteogenic differentiation of human adipose-derived stromal cell (hASC).</p><p><b>METHODS</b>According to the GenBank sequence information of RBP-2, four different small interfering RNAs (siRNA) targeting RBP-2 gene were designed and the corresponding short hairpin RNAs (shRNA) were cloned into pLL 3.7 lentivirus RNA interference vector. The lentivirus with RBP-2-siRNA was packaged in 293T cells. The effective sequence was examined and selected by Western blotting and real-time PCR. The lentiviruses with efficient knockdown effects were used to infect hASC. On the 14th day after osteogenic differentiation, alkaline phosphatase (ALP) activities of hASC were quantitatively tested and at the same time, ALP staining and alizarin red staining were performed to assess the difference of osteogenic differentiation between the knockdown group and the control group.</p><p><b>RESULTS</b>The recombinant lentivirus siRNA targeting RBP-2 was successfully constructed and the expression of RBP-2 mRNA and protein were dramatically suppressed by infection with RBP-2-siRNA lentivirus. On the 14th day after osteogenic induction, ALP activity of hASC in the knockdown group [(299.2 ± 22.7), (224.3 ± 17.7) U/g] was much stronger than that in the control group [(129.9 ± 12.9) U/g, P < 0.05] and the same result was achieved for the ALP staining and alizarin red staining.</p><p><b>CONCLUSIONS</b>The constructed RBP-2-siRNA lentivirus could markedly decrease the expression of RBP-2 and promote osteogenic differentiation of hASC. It indicated that RBP-2 can repress the osteogenic differentiation of hASC.</p>

One-staged surgical treatment of large skin lesions by excision combined with liposuction / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the feasibility of one-staged treatment of large skin lesions by excision combined with liposuction.</p><p><b>METHODS</b>40 skin lesions in 35 cases, which were located at upper arm, thigh, abdomen, lumbus or buttocks, were excised after liposuction at the anatomic sites around the lesion. The wounds were closed by advancing the sliding flaps resulted from liposuction. The operative location was dressed with pressure. The patients were visited on the 7th-14th day, 3rd month and 6th month.</p><p><b>RESULTS</b>All the 40 skin lesions were excised completely and covered with sliding flaps with primary healing. All the flaps survived with no infection, necrosis or wound dehiscence. The skin after liposuction was smooth with improvement of body shape.</p><p><b>CONCLUSIONS</b>The sliding flaps have good mobility after liposuction. The wound after excision of skin lesion can be easily closed by advancing the sliding flaps with good cosmetic and functional results. The lesion should be located at sites with fat accumulation and should not be too large in size.</p>

Effect of liposuction on adipokines, inflammatory markers and insulin resistance / 中华整形外科杂志

<p><b>OBJECTIVE</b>To study the effect of large-volume liposuction on adipokines, inflammatory markers and insulin resistance and its significance.</p><p><b>METHODS</b>The levels of serum adiponectin, resistin, IL-6, TNF-alpha, CRP and insulin sensitivity were measured pre- and 3 months postoperatively in 16 consecutive patients undergoing liposuction.</p><p><b>RESULTS</b>The adiponectin postoperative [(5.56 +/- 1.03) ng/ml] was significantly higher than the preoperative [(6.54 +/- 1.27) ng/ml] (P<0.05); the resistin postoperative [(6.30 +/- 3.25) pg/ml] is significantly lower than the preoperative [(5.18 +/- 2.28) pg/ml] (P<0.05); the insulin sensitivity postoperative (-3.78 +/- 0.39) significantly increased compared to the preoperative (-3.48 +/- 0.32, P<0.05); the TNF-alpha postoperative [(0.19 +/- 0.02) pg/ml] significantly increased compared to the preoperative [(0.23 +/- 0.03) pg/ml, P<0.01]; the postoperative IL-6 [(0.27 +/- 0.04) pg/ml] was similar to the preoperative [(0.31 +/- 0.06) pg/ml, P >0.05]; the postoperative CRP [(8.03 +/- 6.01) mg/L] was not significantly different with the preoperative [(7.28 +/- 6.21) mg/L, P>0.05].</p><p><b>CONCLUSIONS</b>Large-volume liposuction has good effect on insulin resistance and adipokines.</p>

Effect of liposuction on insulin sensitivity and lipid metabolism / 中华整形外科杂志

<p><b>OBJECTIVE</b>To study the effect of liposuction on insulin resistance and lipid metabolism.</p><p><b>METHODS</b>The levels of serum triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and insulin sensitivity were measured pre-and 2-4 months postoperatively in 20 consecutive patients undergoing liposuction.</p><p><b>RESULTS</b>Compared with preoperative, the insulin sensitivity increased significantly, the levels of TC and LDL-C decreased after the liposuction procedure.</p><p><b>CONCLUSIONS</b>Liposuction may improve the insulin resistance and lipid metabolism.</p>

Application of liposuction in scar repair / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore the application of liposuction in scar repair.</p><p><b>METHODS</b>Liposuction using tumescent technique was performed at the scar region. While the local skin was relaxed and tension decreased, scar resection and wound closure followed. The indications and key points of this operation are discussed in the article.</p><p><b>RESULTS</b>This method has been used in 27 patients for scar repair since 2000. The biggest area of excised scar was 40 x 9.5 cm. The width of the excised scar reached 11 cm. All the patients got satisfactory results.</p><p><b>CONCLUSION</b>The operation was simple and safe with little blood loss. After liposuction, a larger area of scar at a fatty region could be excised, and meanwhile, body sculpture was achieved.</p>